Ecological informatics is a multidisciplinary framework for designing sustainable modeling using intensive spatial data, ecological footprint, and computational technology. This study reviews the past literature to identify the ecological networks influencing land-use change and their impacts on natural habitat and biodiversity of study area. Habitat change and loss of biodiversity are the major challenges for sustainable development and UN goals, particularly in the least developing states. The phenomena of dependency of human on natural resources are accelerating in thickly populated cities and creating complicated ecological relationship with mixed morphological characteristics, which need to address urban resilience and earth biocapacity. This past research considered the physical, ecological, social, and environmental constraints as ecological networks to identified eco-nature of area to design ecological models by using ecological informatics for urban managers and government institutions for conservation strategies and their effectiveness at a different scale. These strategies will drive the principles to mitigate the negative impacts of land-use change on biodiversity. The urban managers, land-use planners, policymakers, land managers, and relevant stakeholders use these policies in decision-making for society’s well-being and urban justice.