Introduction: This study introduces a practical-theoretical methodology to train left-handed dentistry students in using their non-dominant hand. This approach addresses training deficiencies that disregard biological factors like cerebral dominance, which result from abrupt changes in laterality and right-handed equipment designs, causing emotional and musculoskeletal challenges. Objective: To validate a practical-theoretical methodology designed to train left-handed dentistry students in using their non-dominant hand, enhancing their acquisition of essential practical skills. This methodology, validated through a Delphi method involving experts across three dimensions (brain dominance, pedagogy, and dentistry), demonstrated strong consensus (W = 0.750) after two rounds of refinement, indicating its potential for effective implementation. Methods: Technological development study in which 15 professionals selected through expert criteria participated, calculating their competence. The methodology was validated through the Delphi method, determining consensus with the Kendall W Test for a 5% significance level (p < 0.05). Results: Two rounds were conducted, gathering expert evaluations and modifying the methodology validation instrument based on suggestions. The methodology to train the non-dominant hand in the Dentistry career was conceived and validated according to the proposed objective. Conclusions: The experts considered the methodology valid, highly useful, and satisfactory, with the potential to gradually transform students’ laterality.