This paper presents a comprehensive, actionable framework for managing innovation and entrepreneurship ecosystems, developed using the Design Science Research (DSR) methodology. The framework is validated through real-world application in an industrial and technologically emerging region, demonstrating its effectiveness in aligning the roles of various ecosystem actors—such as academia, government, industry, and service providers—across the stages of the innovation density funnel. By integrating macro theories such as Collective Impact Theory, Innovation Ecosystem Theory, Resource-based View, and Complex Systems Theory, the framework offers a holistic, structured approach that ensures coordinated efforts among stakeholders, leading to measurable and sustainable outcomes. The framework addresses a critical gap in existing literature by providing both actor-based and activity-based perspectives, integrating them into a cohesive model that facilitates collaboration and resource alignment. It also introduces practical tools for assessing progress, identifying gaps, and setting strategic priorities, offering stakeholders a replicable tool for ecosystem development and scaling. The results of the framework’s application highlight its utility in advancing ecosystem coordination, driving innovation, and fostering economic growth. This study contributes significantly to the field by bridging the gap between theory and practice, offering a model that can be adapted to diverse regional contexts.