The agri-food supply chain faces continuous challenges due to global disruptions, such as natural disasters, market volatility, and rapid technological advancements. These disruptions highlight the vulnerabilities in food production and distribution, emphasizing the need for more resilient and agile supply chain models. The transition from Supply Chain 4.0, driven by automation and technological efficiency, to Supply Chain 5.0, which incorporates human-centric approaches, offers new opportunities to enhance agility and resilience in the agrifood sector. By employing a fuzzy AHP model, this study prioritizes key factors influencing agility and resilience in the agri-food supply chain, validating its consistency through expert surveys. The study evaluates resilience, agility, and the transition from SC4.0 to SC5.0, identifying Risk Management Culture, Demand Management and Enhanced Information Exchange as the most effective strategies, respectively. Finally, this document highlights that by prioritizing demand management and risk culture management, organizations can not only anticipate disruptions but also adapt swiftly, ensuring a transformative shift from technology-centered efficiency to a resilient, human-centric supply chain.