Ultrasound-assisted extraction (UAE) is an innovative technique for protein recovery, offering enhanced yields and improved functional properties. Ultrasound technology enhances protein extraction efficiency by breaking down the structural molecules and facilitating solvent penetration, resulting in higher recovery rates. This approach significantly improves the functional properties of proteins, reduces the anti-nutritional factors and enhances the nutritional quality of extracted proteins. Various studies highlighted in the chapter demonstrate the effectiveness of ultrasound assisted extraction across diverse plant sources, particularly as a pre-treatment. While ultrasound assisted extraction is a promising solution for addressing global protein demands, challenges such as process optimization, industrial scalability, reducing energy requirements, exploring hybrid methods combining ultrasound with enzymatic or microwave-assisted techniques, employing solvent recycling systems, ensuring zero waste concept, etc. remain to be worked out to promote sustainability of the technique.