Public speaking anxiety significantly affects many individuals, particularly students, impairing their ability to deliver effective presentations in academic settings. Virtual Reality (VR) has emerged as an effective tool for creating versatile environments that help users improve their public speaking skills and alleviate associated anxiety. In this study, we developed a virtual reality training system specifically designed to enhance students’ presentation skills and assist them in overcoming public speaking anxiety. This VR environment simulates real-life presentation scenarios and includes a responsive virtual audience that reacts to the real-time presentation. We evaluated the system’s performance through a user study, during which each participant interacted with the system multiple times. Biometric measurements such as heart rate, eye movement, and hand movement were used to assess user performance and responses during their presentations. Additionally, we employed the System Usability Scale (SUS) and the Vertigo Questionnaire (VQ) to gauge users’ subjective evaluations of the system. The results indicated significant improvements in biometric measurements after three training sessions. Participants in the VR simulation training reported increased confidence, reduced anxiety levels, and noticeable advancements in their public speaking skills. Providing a safe and controlled environment enabled learners, particularly students, to enhance their public speaking abilities, address their anxiety, and improve their academic and professional performance.