Virtual reality perspective taking (VRPT) has been shown to be effective in supporting and promoting positive perspective transformation. Building on and expanding prior work, we investigated the mediating role of outgroup perspective taking in the effect of outgroup affinity in VRPT on social acceptance of the outgroup by ingroup members. Our experimental study engaged participants in a VR simulation centered around a Muslim American woman navigating scenarios emblematic of discrimination. Results showed that outgroup perspective taking partially mediated the effect of outgroup affinity on ingroup acceptance of the outgroup member. This indicates that outgroup affinity in VRPT can increase outgroup perspective taking, which, in turn, is associated with increased ingroup acceptance of the outgroup member. These findings highlight the importance of perspective taking in reducing intergroup biases and provide unique opportunities for future research into the use of VRPT for studying psychological phenomena related to ingroup-outgroup biases.