Excessive global petroleum consumption continues to be a primary cause of soil contamination, primarily due to the introduction of toxic fuel components such as benzene, toluene, ethylbenzene, and xylene (collectively known as BTEX) into the environment. Bioremediation techniques can play a crucial role in mitigating this contamination by optimizing microbial activity through alterations in physical and chemical conditions in subsurface environments. This study delves into various strategies aimed at enhancing soil remediation. BTEX-degrading microorganisms were isolated from wastewater treatment plant sludge and subsequently employed as inoculants. These microorganisms underwent an acclimation process within granular activated carbon (GAC) columns, where aromatic hydrocarbons served as their sole source of carbon and energy. Batch experiments were conducted to investigate the augmentation of BTEX biodegradation through the addition of inoculum, nutrients, and oxygen, with parallel control experiments to account for abiotic losses. The findings revealed that, under natural conditions, the degradation of all aromatic hydrocarbons was around 95%; the duration was 45 days and the initial BTEX concentration was 10 mg/L. BTEX utilization rates were calculated and compared across different conditions. The introduction of supplemental nutrients significantly improved the rate of biodegradation, and when hydrogen peroxide was added alongside nutrients, utilization rates increased by 50%. The combined addition of nutrients and microorganisms boosted the rates by over 100% for toluene, ethylbenzene, and xylene. Optimal conditions for the biodegradation of all BTEX compounds were achieved when supplemental oxygen, nutrients, and inoculum were introduced, leading to a more than twofold increase in the rate constants of utilization. Notably, the addition of nitrate proved highly effective in enhancing the degradation rates of toluene, ethylbenzene, and xylene, allowing the degraders to shift to an anaerobic pathway when oxygen was depleted. It was observed that the addition of inoculum expedited the onset of measurable biodegradation. Over time, the indigenous population developed the necessary catabolic abilities and reached a density at which the disparity in degradation abilities between indigenous and preselected biomass was no longer discernible.