Human platelet lysate (hPL) is a regenerative medicine that is widely used in clinics for treating chronic wounds. However, due to the poor understanding of the functions of each protein components, some patients have responded poorly to hPL treatment. Serglycin (SRGN) is present abundantly in platelet granules, and yet the function of the protein in chronic wound healing remains elucidated. Since monocytes shares the same cell lineage as platelets, total mRNA extracted from human monocytic cell line (THP-1) were served as the RNA template for recombinant human SRGN (rhSRGN) expressions. After the recombinant expression vectors were successfully constructed, the vectors were transfected into HEK293T cells for subsequent protein expressions. The expressed rhSRGN were secreted into the conditioned media by the transfected cells. Due to the presence of a hexahistidine (6X His) tag on its C-terminal, the rhSRGN were purified from the conditioned media with an immobilized metal affinity column (IMAC). Western blotting analysis revealed the rhSRGN were decorated with three types of glycosaminoglycan (GAG) chains, heparan sulfates (HS), chondroitin sulfates (CS) and dermatan sulfates (D). In an in vitro assay that mimicked diabetic wound healing, the addition of 10 ng/mL of rhSRGN were showed to significantly enhance the migration of human keratinocytes under hyperglycemic condition.