Harnessing Nanotechnology for Enhanced CRISPR-Cas Plant Genome Editing
摘要
The advent of CRISPR-Cas technology has brought a revolution in plant genome editing, offering unparalleled precision and efficiency in genetic modifications. However, the challenge of effectively delivering CRISPR-Cas components into plant cells persists. This chapter investigates the innovative use of nanotechnology to enhance the CRISPR-Cas system for plant genome editing. We explore various nanomaterials, such as metal nanoparticles, carbon-based nanomaterials, and polymeric nanoparticles, used for delivering CRISPR-Cas components. By examining the mechanisms of nanoparticle-mediated delivery, we highlight how nanotechnology can improve the uptake, stability, and target specificity of CRISPR-Cas components, thereby minimizing off-target effects and enhancing overall editing efficiency. This comprehensive review aims to shed light on the synergistic relationship between nanotechnology and CRISPR-Cas, highlighting its potential to shape the future of plant genome editing.