Exploitation of natural sweeteners has been the prime focus of the consumers for attaining good health mainly for those suffering from diabetic problems. Honey, well known for its countless medicinal values, is appropriate for its use as a natural healthy sweetener. However, the greediness of several food industries or honey producers has led to malpractices, resulting in adulterated honey and labelling them as pure honey or as counterfeit barcodes. Taking cognizance of the adulteration, several regulatory and food safety agencies around the world have set up various parameters for defining the pure honey quality. Numerous high-ended techniques have been used for the detection of adulterants found in honey. Last few years of research have found immunosensors playing an important role in detection of harmful substances in the honey. This chapter discusses the quality of honey, its health benefits along with the role of diverse technologies and immunosensors for checking authenticity of honey.