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Insulin-like growth factor 1 (IGF-1) is important for cellular growth, development, and metabolism. Accurate detection of IGF-1 is crucial for clinical diagnostics and therapeutic monitoring. This paper presents the development of a highly sensitive label-free electrochemical immunosensor using multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO) composite to detect IGF-1. The immunosensor design capitalizes on the exceptional electrical conductivity and large surface area of MWCNT, combined with the unique chemical properties of GO to enhance the immobilization of antibodies and signal transduction. The fabrication process involves the functionalization of MWCNT and GO to form a composite material, which is subsequently deposited onto a screen-printed carbon electrode (SPCE) using the drop-cast method. Anti-IGF-1 antibodies are then immobilized onto the modified electrode surface to capture IGF-1. The immunosensor’s electrochemical performance was assessed using cyclic voltammetry (CV). The study found that the GO-modified SPCE had a CV peak value of Ipa = 246.46 μA, while the CV peak current of GO-MWCNT composite nanomaterials reached Ipa = 458.71 μA, indicating that the composite material exhibits higher electrical conductivity compared to GO. Moreover, the GO-MWCNT composite showed a significant detection of IGF-1. This result suggests that the GO-MWCNT composite platform has great potential for clinical applications in detecting IGF-1 due to its high sensitivity.

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Development of an Electrochemical Immunosensor Based on Multi-walled Carbon Nanotubes and Graphene Oxide Composite for the Detection of Insulin-Like Growth Factor 1

  • 会议论文

  • 发表日期：

    2025年

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  • Zeren Chen,
  • Fatimah Ibrahim,
  • Norrima Mokhtar,
  • Nor Syafirah Zambry,
  • Nurul Fauzani Jamaluddin,
  • Wan Nurazreena Wan Hassan,
  • Wan Ahmad Hafiz Wan Md Adnan,
  • Bojan Petrović,
  • Sanja Kojić,
  • Goran M. Stojanoić

摘要

Insulin-like growth factor 1 (IGF-1) is important for cellular growth, development, and metabolism. Accurate detection of IGF-1 is crucial for clinical diagnostics and therapeutic monitoring. This paper presents the development of a highly sensitive label-free electrochemical immunosensor using multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO) composite to detect IGF-1. The immunosensor design capitalizes on the exceptional electrical conductivity and large surface area of MWCNT, combined with the unique chemical properties of GO to enhance the immobilization of antibodies and signal transduction. The fabrication process involves the functionalization of MWCNT and GO to form a composite material, which is subsequently deposited onto a screen-printed carbon electrode (SPCE) using the drop-cast method. Anti-IGF-1 antibodies are then immobilized onto the modified electrode surface to capture IGF-1. The immunosensor’s electrochemical performance was assessed using cyclic voltammetry (CV). The study found that the GO-modified SPCE had a CV peak value of Ipa = 246.46 μA, while the CV peak current of GO-MWCNT composite nanomaterials reached Ipa = 458.71 μA, indicating that the composite material exhibits higher electrical conductivity compared to GO. Moreover, the GO-MWCNT composite showed a significant detection of IGF-1. This result suggests that the GO-MWCNT composite platform has great potential for clinical applications in detecting IGF-1 due to its high sensitivity.