The supply chain of seafood products creates a large amount of waste, from fishing discards to by-products of industrial processing. This biomass contains however valuable materials with potential in tissue engineering and regenerative medicine. The present chapter focuses on the recovery of biopolymers from seafood waste with applications in this field from the point of view of sustainability in a broad sense. Such perspective allows in the first place the identification of raw materials suitable for the isolation of the biopolymers of interest, based on their abundance as by-products and discards, and leaving aside endangered species. Conversely, this selection narrows the number of biopolymers amenable to sustainable production, which concentrate on chondroitin sulfate, chitin, chitosan, collagen and gelatin. And secondly, it provides the scope to analyse how green are the most widely used valorization processes to recover each biomaterial, proposing cleaner alternatives and outlining steps towards their integral valorization by finding uses for the effluents stemming from the isolation processes. This ultimately contributes to the viability of the valorization processes and places seafood production closer to the zero-waste goal.