The dynamic performance of footbridges under pedestrian loading significantly impacts pedestrian comfort. The current design guidelines primarily use the structure acceleration to evaluate comfort and therefore neglect the contribution of other influential factors. This study investigates the influence of pacing frequencies as well as structural peak acceleration on subjective ratings of vertical vibration comfort on four footbridges. The acceleration limits specified by Sétra, BS 5400, and ISO 10137 were also critically evaluated. The effect of the parameters above on the subjective comfort were then statistically analysed. It was found that the subjective ratings varied significantly across different bridges within the same Sétra’s vibration limit. Similarly, BS 5400 and ISO limits showed that nearly 50% of participants remained happy with perceived vibrations even when these limits were exceeded. The statistical analysis revealed that both peak acceleration and pacing frequency play critical role in shaping pedestrian comfort. Overall, it was found that pedestrians may become less sensitive to vibration at higher pacing frequency, reducing their perception of discomfort and improving comfort levels under high acceleration conditions. These findings provide insights into the need to include pedestrian pacing frequency along with structure peak acceleration into vibration serviceability evaluation of footbridges to ensure optimal pedestrian comfort.