Biomaterials have undergone substantial development in regenerative medicine, beginning as inert supports and progressing to bioactive materials that drive tissue regeneration. The purpose of these biomaterials is to imitate the natural extracellular matrix to encourage cell adhesion, migration, proliferation, and differentiation. There has been a change toward an integrated strategy involving material researchers, biology researchers, and physicians, with a primary emphasis on tissue engineering and regenerative medicine, according to historically significant perspectives. They play an essential part in bone tissue engineering by facilitating bone regeneration through various scaffolds, including hydrogels, nanofiber scaffolds, and composite scaffolds printed using 3D printing technology. To facilitate cell proliferation and differentiation to replicate the extracellular matrix, scaffolds are synthesized from natural or synthetic polymers to create biomaterials for use in tissue engineering. Developing highly biodegradable frameworks that include antimicrobial qualities is now a topic that is being pursued to accelerate tissue regeneration and prevent infections. The multidisciplinary advancements in biomaterials continue to propel innovation in regenerative medicine. These advancements highlight the significance of cell-biomaterial interactions and the harmonious degradation of biomaterials for the successful regeneration of tissue. To summarize, the development of biomaterials in regenerative medicine represents a multidisciplinary strategy intending to develop implants that speed up the process of tissue regeneration. This chapter will provide the perspectives on biomaterials in regenerative medicine.