In the field of tissue engineering and regeneration, biomaterial-based additive manufacturing (AM) has shown great promise in providing accurate scaffolds with specific features. The difficulties and potential applications of biomaterial-based AM in tissue engineering and regeneration are covered in this chapter. The difficulties of recreating the natural tissue microenvironment, the lack of biocompatible materials appropriate for printing, and the requirement for faster and more precise printing techniques are some of the difficulties associated with biomaterial-based additive manufacturing. Innovations in material science, bioink creation, and printing technologies are necessary to overcome these obstacles. The creation of novel biomaterials with improved biocompatibility and bioactivity, the integration of additive manufacturing with other bio fabrication methods (such as bioprinting and electrospinning), and the application of innovative imaging methods for patient-specific scaffold design are some future directions for biomaterial-based AM in tissue engineering and regeneration. Furthermore, the fabrication of functional tissue constructs for drug screening and transplantation offers considerable potential when biomaterial-based AM is combined with stem cell technologies and bioreactor systems. Translating these advances from the lab to clinical practice will need cooperation between investigators, medical professionals, and regulatory agencies. In conclusion, by overcoming present obstacles and pursuing novel avenues, biomaterial-based AM can transform tissue engineering and regeneration. Biomaterial-based AM can lead to better patient outcomes and individualized regenerative medicine by tackling these issues and looking toward the future.