This study examines the perceptions and knowledge of 122 staff nurses regarding Quality Management Systems (QMS) in a tertiary hospital, with a focus on insights for effective knowledge management in healthcare. Personal factors such as age, gender, education, and employment length were analyzed for their influence on QMS understanding. Results revealed a generally positive perception of QMS and highlighted key knowledge areas, including strengths in Continuous Improvement and Process Approach, as well as gaps in Relationship Management. Female nurses demonstrated higher QMS knowledge than males, and higher educational attainment correlated with better comprehension. These findings reflected the need for targeted knowledge management strategies to address gaps, particularly in Relationship Management, fostering more effective adoption of QMS principles. Enhancing knowledge-sharing mechanisms and training programs can improve engagement with QMS, ultimately advancing patient care and operational efficiency. Future research should explore longitudinal approaches to deepen understanding of QMS knowledge dynamics in healthcare settings.