This research paper explores the efficacy of an innovative ITK (Indigenous Technology Knowledge) based chili-neem biopesticide in targeting common sucking pests infesting vegetable crops. The biopesticide's strategic focus on sucking pests necessitates a targeted approach to pest management, utilizing potent ingredients such as green chili extract, red chili extract, neem leaf extract, molasses, garlic, and cow urine to enhance efficacy. The effect was evaluation firstly by brine shrimp lethality assays to assess the lethal dose. The most effective lethal dose of 7–10% were further evaluated through laboratory trials on pests such as aphids, mealy bugs, and hadda beetles demonstrates the concentration-dependent effectiveness of biopesticides in controlling pest populations. Overall, ITK-based chili-neem biopesticide is a novel innovative Agro product promising a sustainable alternative for managing pest infestations in vegetable crops, aligning with the principles of eco-friendly and healthy agriculture.