The disk diffusion method is one of the most frequently techniques to determine the pathogens antibiotic susceptibility. A fast identification of resistance profiles is essential for researchers and physicians. In laboratories without technological resources these measurements are done manually using a ruler or a caliper increasing the chances of error. We have designed a device that by means of image processing of disk diffusion tests semi-automatizes the process. A total of 867 halos in 95 culture plates were evaluated by comparing an expert’s naked eye reading with the AntibiHórus system measurement and a correlation coefficient (r) of 0.979 was obtained between the readings. The developed system proved to be user-friendly in the routine of the HCPA microbiology laboratory. After approximately 1 month using the two systems in parallel, the AntibiHórus system definitively replaced the original equipment (Bio-Rad Osiris \(^{\circledR }\) ).