Electroporation, a process that uses Pulsed Electric Fields (PEF) to increase cell permeability, holds significant promise for various biomedical applications, including drug delivery, gene therapy, and cancer treatment. However, accurately monitoring cellular responses to PEF remains a challenge. Impedance is a indirect way to monitor cell poration. The \(\beta \) -dispersion is affected by electroporation. In this study, we investigated the use of square wave and fast Fourier transform to calculate impedance. We assess the impedance on ex vivo liver tissues before and after to PEF. We calculate impedance in the frequency spectrum 1 kHz to 21 kHz. We validate our method using traditional impedance spectroscopy. We detect a current increases of 27% and impedance magnitude decreased approximately 50% after PEF. The ability to integrate impedance sensing with PEF techniques offers an alternative approach to monitor PEF treatment and study electroporation.