The Yamuna River in Delhi, India, serves as a critical water resource for both human consumption and aquatic ecosystems. This study investigates the monthly site-wise variations in zinc (Zn) concentrations within the river over the course of a year. The primary objectives were to assess the dynamics of Zn pollution, identify potential pollution sources, and evaluate the associated health and environmental implications. Five strategic sampling sites along the river were selected to represent various aspects of river usage and environmental conditions. Water samples were meticulously collected each month, spanning an entire year, and underwent rigorous analysis. The results unveiled a complex interplay of factors contributing to Zn pollution in the river. Site-specific variations indicated localized pollution sources along the river's course, warranting further environmental forensics investigations. Additionally, the observed fluctuations in Zn levels throughout the year underscored the presence of seasonal pollution patterns influenced by climatic conditions, agricultural practices, industrial processes, and urbanization. Most significantly, the study revealed that Zn concentrations consistently exceeded the World Health Organization's (WHO) permissible limits for drinking water at multiple sampling sites. This alarming trend raises significant health concerns for communities reliant on the Yamuna River's water resources. Furthermore, elevated Zn levels pose threats to aquatic life and the overall ecological health of the river, necessitating habitat restoration and conservation efforts. The study's implications extend to policymakers and regulatory bodies, emphasizing the urgent need for stringent water quality regulations and pollution control measures to safeguard the Yamuna River's water resources. This research lays the groundwork for future investigations, including pinpointing pollution sources, long-term monitoring, expanding the scope to include other contaminants, and developing effective mitigation strategies. In conclusion, this study sheds light on the critical issue of Zn pollution in the Yamuna River, emphasizing the need for immediate action, multidisciplinary collaboration, and sustained monitoring to ensure the long-term sustainability of this vital water resource and the ecosystems it supports.