The integration of Artificial Intelligence Generated Content (AIGC) into cultural promotion has gained significant attention, particularly on platforms like Douyin and Bilibili. AI-generated micro-web dramas utilize intelligent content creation and personalized recommendations to expand the reach of traditional culture. However, replicating the emotional depth of traditional culture remains a challenge, with user acceptance and psychological responses playing a critical role in effectiveness. This study applies “Uses and Gratifications Theory” to examine user perceptions of AI-generated cultural content, exploring emotional reactions, acceptance factors, and demographic influences. The findings provide theoretical insights and practical strategies for advancing AI-driven cultural communication.