Microbially Induced Calcium Carbonate Precipitation (MICP) has recently emerged as an environmentally friendly technique with various applications, including water remediation, soil quality improvement, oil and gas engineering, and the repair and maintenance of cementitious construction materials. Although MICP has been observed in many groups of microorganisms, most research to date has focused primarily on bacteria, with other microbial representatives largely overlooked. This study, therefore, aims to investigate the potential of yeasts isolated from construction materials to produce CaCO₃, with the purpose of enhance the quality of demolition waste for future reuse. Using classical microbiological methods based on a selective medium supplemented with calcium acetate, three distinct colonies differing in color and morphology were selected and labeled as LCEQ1, LCEQ2, and LCEQ3. Each yeast isolate was then individually inoculated into 500 mL Erlenmeyer flasks containing 100 mL of a production medium optimized for CaCO₃ precipitation. All flasks were agitated at 150 rpm at 30 °C for 7 days, with a control flask without microbial inoculum maintained under identical conditions. After incubation, a white precipitate was visibly observed in all yeast-inoculated flasks, and FTIR analysis confirmed the presence of CaCO₃. No precipitate was observed in the control flask. The amount of calcium carbonate was measured through thermogravimetric analysis. Among the three isolates, yeast LCEQ2 demonstrated the highest acetate-to-carbonate conversion (56.3%), followed by LCEQ3 and LCEQ1. These findings suggest that concrete aggregates may serve as a promising source of yeasts capable of precipitating calcium carbonate.