Enhancing reproductive performance in fish through effective nutritional strategies is essential for sustainable aquaculture and the production of superior offspring. Providing a well-balanced diet rich in key nutrients, including proteins, lipids, vitamins, and minerals, is crucial for improving gamete quality and gonadal development. Vital elements such as omega-3 fatty acids, antioxidants like vitamins C and E, and trace minerals like zinc and selenium offer significant benefits. Additionally, customized broodstock diets, functional feed supplements, and probiotics help optimize reproductive success by promoting hormone production, strengthening immune function, and improving nutrient utilization. By tailoring these nutritional interventions (Fig. 20.1) to specific species and environmental conditions, aquaculture operations can achieve enhanced reproductive performance and greater overall efficiency.