This study aimed to examine the influence of health literacy, presentation format and presentation state on the comprehension of personal digital health information among middle-older aged users. A three-factor mixed design was employed in experiment. Task performance, perception measures and eye movement measures were collected from 25 middle-older aged adults under health literacy (i.e., low, medium and high), presentation format (i.e., bar graph and text format) and presentation state (i.e., static presentation and dynamic presentation). Repeated measures analyses of variance were used to analyze experiment data. The results showed that high health literacy resulted in a higher accuracy rate. Bar graph made fewer errors compared to text format. Although dynamic presentation was not perceived as easier as static presentation, it led to a longer total fixation duration. Presentation format significantly interacted with presentation state on perceived ease of comprehension and total fixation duration. However, they may also limit focus to localized information, hindering understanding of the broader context.