This research investigates the mediating influence of intrinsic motivation on employee isolation and job performance of work-from-anywhere Information Technology professionals in India. Intrinsic motivation and ethical integrity are fundamentally intertwined, each enhancing and reinforcing the other. The lenses of the self-determination theory underpin the study. The research questions covered in the study are: (a) Does employee isolation affect job performance? (b) Does intrinsic motivation during employee isolation affect job performance? (c) Does intrinsic motivation mediate the connection between employee isolation and job performance? The SEM based approach survey was conducted to collect data from 410 IT employees who work from anywhere for a minimum of one or more days per week. The findings indicate that (a) employee isolation has a negative influence on job performance, (b) intrinsic motivation significantly influences job performance during employee isolation, and (c) intrinsic motivation mediates indirect-only (full mediation) the link between employee isolation and job performance. The employers need to build upon intrinsic motivation tools for work-from-anywhere Information Technology professionals, which ensures employees’ relatedness by giving rewards, a sense of purpose towards assignments, autonomy through intrinsic motivation and ethical integrity, and developing IT expertise, which significantly increases performance among employees in work isolation situations. Through the lens of self-determination theory, the research contributes to the work-from-anywhere literature by exploring the relations between employee isolation, job performance, and the mediating influence of intrinsic motivation on IT professionals in India in the current scenario.