Ethnobotany assumes great importance in enhancing our knowledge about plants used by ethnic and tribal community, and their traditional methods adopted for conservation of bioresources. Tribes through their own observation and experience developed knowledge about the usage of plants for edible, medicinal and other purposes. The present investigation has been undertaken with Kattunaickan tribes from Janakikkadu forest, Kozhikode district, Kerala. The study provides 80 plant species belonging to 39 families which include both wild and cultivated plant species utilized by the tribes for food, medicine and ethnoveterinary medicine. The family Fabaceae registered most dominant species followed by Asteraceae, Rutaceae and Euphorbiaceae in their use. From life-form analysis, herbs were found to be dominant, and subsequently, shrubs, trees, climbers, ferns and epiphytes were recorded. Among utilization of plant parts investigated, leaves were commonly used for the treatment of cough, cold, jaundice, urinary infection, rheumatism. The species like Rauvolfia serpentina, Gloriosa superba, Curculigo orchioides were reported as threatened plant species, while Aegle marmelos, Lagerstroemia microcarpa, Glycosmis pentaphylla were found as endemic plant species to the region. Being an important group of plants of high exotic and aesthetic value, plant species need immediate protection as their population are dwindling.