Biofilms are complex, multicellular communities of microorganisms enclosed in a self-produced extracellular matrix. These structures play a pivotal role in chronic infections, industrial biofouling, and the growing challenge of antibiotic resistance. Advances in high-throughput technologies have revolutionized biofilm research by enabling the application of “omics” methodologies—genomics, transcriptomics, proteomics, and metabolomics, providing powerful insights into the molecular mechanisms underlying biofilm behavior. This chapter explores the latest omics-based strategies used to study biofilms, highlighting their role in uncovering the intricacies of biofilm formation, persistence, and resistance. It also examines the applications of these approaches in both medical and industrial settings. Furthermore, this chapter discusses future directions for omics research in biofilm biology, emphasizing the transformative potential of integrating multiomics datasets to gain a holistic understanding of biofilm dynamics and their broader implications. This chapter mainly focuses on the role that phenomics plays in deciphering the intricate relationships between biofilm production, structure, and function. In both natural and artificial settings, biofilms organized populations of bacteria embedded in an extracellular matrix that they produce themselves are common and have an impact on a variety of bacterial functions. Phenomics allows researchers to study biofilm dynamics, including microbial adaptation, communication, and responses to environmental stressors, through sophisticated phenotypic profiling. This chapter examines the use of data-driven methodologies, imaging methods, and high-throughput phenotyping tools to analyze biofilm behavior and its consequences for medical interventions, bioremediation, and antibiotic resistance. Novel approaches and their potential to address urgent issues in microbial ecology, biotechnology, and public health by incorporating phenomics into biofilm research were also discussed.