In order to ensure the normal operation of the equipment in the man-machine interactive computer room, a remote intelligent inspection method of the man-machine interactive computer room based on digital twinning technology is proposed. Collect information data in that remote intelligent inspection process of the man-machine interactive machine room, standardize and unifying the information in the environmental inspection process, fitting the characteristic data of the signal information, the equipment information and the environmental information of the man-machine interactive machine room, and obtain the collection result of the remote intelligent inspection information data of the man-machine interactive machine room. According to the spatial distribution value, the change of standard temperature value is calculated, and the digital twin technology inspection model is constructed to realize the remote intelligent inspection of man-machine interactive computer room. The experimental results show that this method has good inspection effect, can ensure the accurate tracking of driving direction and angle, and has high inspection accuracy.