The characterization of DNA through its electrical properties represents a technique with enormous potential but has so far been insufficiently explored. Developing biosensors capable of detecting DNA is a significant technological challenge of great relevance for advancing biotechnology and medicine. In this context, our research group has addressed this challenge by using multifrequency bioimpedance measurements, a technique we have identified as a promising tool for estimating total DNA concentrations in biological samples. Through an innovative approach, we have incorporated magnetic nanoparticles in the measurement process, enabling a more accurate and efficient characterization of DNA. The results not only provide solid evidence on the efficacy of this technique but also open new perspectives for the application of nanotechnology in biomolecule characterization. This study suggests that combining bioimpedance and nanotechnology can revolutionize how DNA characterization is approached, offering a non-invasive, relatively fast, highly sensitive method with applications that could extend to diverse areas, from clinical diagnostics to advanced genomic research.