Determination of human genomic Deoxyribonucleic Acid (DNA) is an essential procedure in molecular biology studies. However, specialized equipment for this purpose is not always available in resource-limited settings. Development of innovative, cost-effective, and easy-to-use devices for estimating DNA concentrations remains a relevant technological challenge. Electrical Bioimpedance Spectroscopy (EBiS) is a relatively simple and low-cost technique for exploring the electrical properties of biological analytes, and has been previously proposed for DNA quantification. In this study, the use of EBiS based on the EVAL-AD5933EBZ is proposed as a low-cost and easy-to-use technique for detection of DNA concentrations. A small sample size (n = 10) with DNA concentrations within a useful range for molecular biology assays (10 to 25 ng/μL) was used as proof of concept. EBiS measurements trough the AD5933EBZ in the range of 5 to 50 kHz were compared to a calibrated multifrequency bioimpedance meter as reference parameter to determine a percentage error. EBiS magnitude values showed a reduction in error with increasing frequency. In contrast, an increased error for phase values is shown as a function of frequency. The EVAL-AD5933EBZ showed a relatively low and potentially acceptable percentage error (±10%) for magnitude and phase values in the selected frequency range.