The development of non-invasive light microscopy-based technologies for live cell imaging is always a compromise between the careful task to keep the cell alive for a long period of time and to acquire the highest resolution image for further analysis. An innovative technology based on spectral light analysis was developed for high-resolution live cell imaging combining light microscopy with sophisticated computational and visualization software. This enables analysis of dynamic intracellular processes within an unlabeled cell as well as the interaction and communication of cells with each other. The visualization is based on calculating spectral light characteristics (reflectance) for each pixel of a dataset within the image from the spectra of the microscope camera filters and light source. The technical advantage for the user is a large field of view on the microscopic image with many cells to analyze as single cells or in the tissue context. This new technology enables the analysis of unstained living cells over time without compromising their functional capability to expand, interact, communicate, or react to pharmacological agents, which makes this analytic method suitable for diagnostics in the field of precision medicine.