Engineered tissues are large structures made up of different cell types within a three-dimensional scaffold mimicking the extracellular matrix. Over days and weeks in culture, the cells establish tissue-like cell-cell and cell-matrix contacts forming complex internal structures with an inhomogeneous refractive index. Because of the refractive index mismatch, bioengineered tissues strongly scatter light as their native counterparts and are optically opaque at most wavelengths used in light microscopy. Moreover, large bioengineered samples are challenging to image with fluorescence microscopy due to their size and the time required to record their full volume. This also relates to the fact that most microscopes waste the sample’s available photon budget—the quantity of photons that the sample can emit before deteriorating—by exposing it to high-intensity light that induces photodamage and photobleaching. Fluorescence imaging is particularly affected by photon wasting. Among the available fluorescence microscopies, light sheet fluorescence microscopy (LSFM) is optimally suited to image large three-dimensional specimens. LSFM drastically reduces photodamage by placing the excitation and detection optics orthogonally and not collinearly, at variance with confocal microscopy. Besides volumetric fluorescence imaging, also light-based three-dimensional (3D) bioprinting techniques can greatly benefit from light sheet illumination as the improved photon budget and speed of light sheet microscopy can be leveraged in these bioprinting processes. The lower light dose combined with fast patterning ensure higher cell viability in the final constructs. A further benefit of the light sheet bioprinter is that three-dimensional imaging of the constructs can be performed on the same device. In fact, the cells need to be imaged both during printing and post-printing to monitor their collective self-organization in the bioengineered tissue. Using fluorescent probes, assessment of bioprinted samples can be performed following a straightforward workflow. This chapter present a short summary on the application of light sheet technology for imaging and bioprinting of bioengineered samples. It also presents an overview of its application in the bioengineering field, including work on soft 3D bioprinting and 3D patterning of tissue constructs.