The integration of blockchain technology into agri-food supply chains offers transformative opportunities to enhance transparency, traceability and trust among stakeholders. This chapter delves into blockchain implementation and its benefits through four Australian case studies. These firms utilize blockchain to streamline documentation, enhance product evaluation and automate food recall processes, effectively addressing consumer demands for greater transparency and traceability. Transitioning from traditional to digital processes, along with the tokenization of assets and events, ensures the reliability and verifiability of supply chain data. However, challenges such as scalability, data privacy and supplier compliance remain crucial barriers. To address these issues, strategies include partnering with technology providers, integrating public blockchain networks and embedding conditional token minting within commercial workflows. Likewise, fostering collaboration among stakeholders and investing in scalable blockchain solutions are imperative for overcoming these challenges. This chapter underscores blockchain’s pivotal role in transforming agri-food supply chains, highlighting its potential to enhance food safety and sustainability. It calls for continued exploration and widespread adoption of blockchain technology to ensure transparent and efficient agri-food systems.