Globally, the rising number of herbicide-resistant weeds is a consequence of the dissipation and overuse of herbicides, as well as insufficient unique active ingredients. Furthermore, the challenges in managing weeds are made worse by weed characteristics that support the persistence of weed seed banks. Therefore, molecular control of weeds is an applied science that includes constant and continuous development of molecular genetic techniques that reduce reliance on herbicides while managing stubborn weed species. Weed management–related technologies developed through molecular biology include molecular genetic analysis, genetic transformation, finding and isolating genes of interest, and introducing “suicide” genes into plants of interest. Moreover, molecular biology has been utilized to understand more about how weeds endure adverse environmental conditions, engage in competition and interaction with nearby plants, and develop resistance to the herbicides employed to control them. Whether through increasing crop-weed competitiveness for space, light, nutrients, and water or via allelochemicals, molecular biology should be taken into consideration for weed management without the use of or with limited herbicide application. The possibility of controlling weeds by direct genetic manipulation has become prominent with the introduction of CRISPR/Cas9 gene-editing tools, as well as recent breakthroughs in “omics” and less expensive sequencing technology, which could be accomplished either permanently or temporarily. The first generation of transgenic products has proven successful, and the massive commercialization effort to use chemical and molecular tools to solve weed control challenges has substantially benefitted crop production. Furthermore, these strategies comprise an array of techniques that could potentially be utilized to alter the crop to boost its competitiveness or resistance to herbicides or to alter the expression of significant genes in weeds to lessen their degree of fitness and effectiveness. Despite knowing that applying these promising molecular technologies to weed control will present considerable practical, legal, and technological difficulties.