AI-powered multi-layered Phishing Defense Framework (AIPDF) is developed in response to the increasing complexity and sophistication of phishing attacks in the digital age. Traditional cybersecurity measures often fail to address these evolving threats, necessitating a more dynamic, intelligent, and multi-faceted approach. AIPDF leverages AI, machine learning, and blockchain technologies to provide comprehensive, real-time defense mechanisms against phishing, ensuring more accurate detection and faster response. The AI-powered multi-layered Phishing Defense Framework (AIPDF) is designed to counter evolving phishing threats by integrating multiple layers of defense. Each layer addresses different aspects of phishing attacks, from real-time anomaly detection to automated incident response. The framework combines AI-powered phishing detection engines, blockchain-based email authentication, and real-time threat intelligence to enhance email security. It also incorporates multi-factor authentication and user training to ensure continuous protection. This system enables rapid detection and response to phishing attacks while providing cybersecurity infrastructure transparency, adaptability, and resilience. Future iterations of the framework can integrate additional AI/ML models and extend to broader cybersecurity challenges.