This study aims to understand the impact of digital entrepreneurship in its dimensions (digital leadership, digital culture, digital innovation, digital environment, and digital financing) on strategic success in its dimensions (survival, growth, and adaptability), considering the mediating role of organizational ambidexterity in Jordanian public universities. A research framework was developed that sheds light on the state of research after a comprehensive analysis of the available literature, and the empirical results of this study were based on a sample consisting of participants, of whom provided responses that were valid for statistical analysis, which relied on the Smart—PLS program. Among the most important findings of this study was the absence of a direct relationship between digital entrepreneurship and strategic success in Jordanian public universities, and the presence of a strong direct relationship between digital entrepreneurship and organizational ambidexterity, while the results showed a strong indirect relationship between digital entrepreneurship and strategic success with the presence of organizational ambidexterity.