Laponite nanoclay is a synthetic trioctahedral smectite with a structure composed of layered silicates amalgamated from inorganic mineral salts. Laponite is already commonly used in cosmetic and biomedical applications because of its unique structural and physical features. Recently, it has been used as an additive agent or main component in drug delivery, hemostat, tissue engineering, cell adhesion, and proliferation applications. In this study, we aim to investigate the electrospun parameters in order to fabricate a polycaprolactone (PCL)/laponite electrospun membrane and characterize its properties such as morphological, wettability, and cytotoxicity characteristics. Laponite nanoclay is added to different ratios of dimethylformamide/acetone solution system, then PCL is added to the solution, and the obtained solution is electrospun by a pilot scale electrospinning machine under the voltage of 40 kv to make PCL/laponite membrane. The physicochemical properties of the fabricated membranes were characterized using scanning electron microscope (SEM), and wettability testing. The cytotoxicity of the obtained membranes is also tested using the resazurin assay. After adding a small amount of laponite, there is a slight increase in fiber diameters and hydrophobicity of the membranes. Different ratios of the solvent system show different morphologies and forms of PCL/laponite membranes after the electrospinning process. Furthermore, the cell viability test indicates that the PCL/laponite membrane is non-cytotoxicity. The results show that PCL/laponite membranes have great potential for biomedical applications.