Introduction: Antibiotic resistance is a significant worldwide problem, including in Vietnam. ESKAPE pathogens are recognized as the primary culprits behind nosocomial infections. To develop an effective resistance management strategy, understanding the characteristics of these microorganisms and assessing their resistance rates is paramount. Objectives: The research aims to determine the prevalence of different types of ESKAPE pathogens isolated at a hospital in Ho Chi Minh City and assess their resistance rates. Methodology: A cross-sectional descriptive study was conducted on all antibiotic susceptibility results of ESKAPE pathogens at the hospital in 2022 (01/01/2022 – 31/12/2022). Result: The study encompassed 2868 positive results for ESKAPE pathogens, accounting for a 75,6% microbiological isolation rate. Escherichia coli (35,9%), Klebsiella pneumoniae (19,7%), Acinetobacter baumannii (15,6%), Staphylococcus aureus (14,2%), Pseudomonas aeruginosa (10,8%), and Enterococcus faecium (3,8%) were the most frequently isolated pathogens. ESKAPE pathogens were prevalent in respiratory samples, constituting 27% of isolates. The multidrug resistance (MDR) rate of Acinetobacter baumannii was 43,5%, exhibiting high resistance to ceftriaxone (94%), ceftazidime (93%), cefepime (93%), imipenem (92%), and ciprofloxacin (93%). Pseudomonas aeruginosa showed an MDR rate of 26,3%. Escherichia coli exhibited ESBL secretion in 68,3%, with 33 cases of gen AMPC, showing high resistance to ciprofloxacin (70%), piperacillin (88%), and ceftazidime (53%). Klebsiella pneumoniae had an ESBL secretion rate of 27,3%, with 8 cases of gen AMPC. Staphylococcus aureus demonstrated MRSA prevalence (65,2%) and susceptibility to vancomycin, teicoplanin, and linezolid. Enterococcus faecium showed resistance to vancomycin (30%) and teicoplanin (3%). Conclusion and Discussion: ESKAPE pathogens, particularly with the emergence of MDR isolates, are prevalent in hospitals, posing significant challenges for physicians in treatment. Clinicians should update hospital antibiotic guidelines based on current local antibiograms to improve the adequacy of antibiotic therapy and address antimicrobial resistance genes.