Oral and maxillofacial bone grafting requires both function and aesthetics. 3D printing scaffolds is the utmost method for bone reconstruction. The Gyroid, a member of TPMS family, are smooth, infinitely extending, with large surfaces areas, zero mean curvatures, good mechanical properties, and mimicry cancellous bone. Polylactic acid (PLA), a biomedical-compatible material, is popularly used in 3D FDM printing but has hydrophobic properties. NaOH treatment of 3D printed PLA scaffolds practical activation without losing mechanical property PLA scaffolds. In this study, 3D printing Gyroid scaffolds were designed with different porosity by FDM method with PLA filament. These scaffolds were immersed in 2M NaOH for 1h for etching their surfaces. Then the characterization tests of morphology, chemical, hydrophilicity, and strength were carried out by SEM, FTIR, contact angle, and compression test, respectively. The FTIR test showed the -OH, -CH functional groups on the Gyroid’s surface. Etching the surface with a proper concentration of NaOH improves the hydrophilicity, keeping the Gyroid geometry and mechanical properties of the scaffolds. Modifying the chemical of 3D Gyroid bone scaffolds could help to improve cell attachment for bone regeneration.