Introduction: Positive effects of non-invasive LED therapy in wound care and aes-thetics are being highly acknowledged and widely applied in medicine. LEDs with diverse wavelengths have been shown to be highly effective in accelerating wound healing, relieving pain and reducing inflammation. The effects of LED have expedited many studies on combining different wavelengths to enhance non-invasive light therapy for patients. The proposed study builded a combined LED therapy device and then conducted experiments to evaluate the effect of the therapy on mice wound model. Methodology: Fifteen five-week-old female Swiss mice were uniformly wound by incising and then divided into 3 groups including: one untreated control group, one treated group with combined wavelengths of 630 nm and 850 nm; and one treated group with a combined wavelength of 630 nm and 940 nm, respectively. The therapy for the treatment group was applied 24 h after wounding with a dose of 5 times per week, and each 10 min exposure for two consecutive weeks. Results: During two weeks of treatment, the wound recovery speed of the two treated mice groups was compared with the control group; and the results showed that the wound healing time of the group exposed to LEDs was about 4 days faster than the control group. Particularly, the therapy group that combined wavelength of 630 nm and 940 nm gave the best results with the ability of blood clotting, wound closure and recovery 1–2 days faster than the one of 630 nm and 850 nm. Discussion and conclusion: Overall, the experiment results showed a good effect on improving wound healing of therapy combined LEDs and the potential of this method for future development.