The scarcity of fully grown oocytes poses a significant challenge to infertility treatments. Thus, developing an In vitro growth system to utilize the abundant and potential source of growing oocytes in the ovary has emerged as a promising approach for assisted reproductive techniques. However, the current efficiency of In vitro growth (IVG) for oocytes isolated from early antral follicles (EAFs) (1–1.5mm) remains notably low. During IVG, oocytes are exposed to oxidative stress, which requires strong transcriptional activity to synthesize essential proteins. L-ascorbic acid (AA) is a well-known antioxidant that reduces oxidative stress caused by reactive oxygen species (ROS). Scriptaid is a compound that inhibits histone deacetylase (HDACi), enhancing transcriptional activity and protein expression. Thus, this study aims to assess the combined effects of AA and Scriptaid on IVG and meiotic competence of oocytes derived from EAFs. Oocyte-cumulus-granulosa complexes (OCGCs) were cultured in IVG media supplemented with varying concentrations of AA (0, 50, and 100 µg/mL) for 4 days. Subsequently, the optimal concentration of AA, determined based on oocyte quality assessments, was combined with Scriptaid at two concentrations (50 nM and 25 nM) for two different durations (10 h and 24 h). Morphology, oocyte survival rate, diameter, antrum formation, and chromatin configuration were evaluated in each treatment. Meiotic competence was assessed following 7 h of pre-In vitro maturation (pre-IVM) and 42 h of In vitro maturation (IVM). The results revealed that IVG medium supplemented with 50 µg/ml AA enhanced oocyte survival rate. Combining AA with Scriptaid for 10 h improved IVG growth efficiency and meiotic competence compared to AA alone, with no significant difference observed between Scriptaid concentrations. In conclusion, the IVG medium containing 50 µg/ml AA combined with 10 h of Scriptaid treatment exhibited enhanced cytoplasmic competence in oocytes and supported oocyte maturation.