The present study presents a novel screw-based continuous extrusion 3D bioprinting head, which has been specifically developed to improve the accuracy and consistency of biofabrication processes. A thorough examination of the existing literature reveals the constraints associated with current 3D bioprinting technologies, prompting the proposal of a screw-based system as a potential solution to overcome these limitations. The system demonstrates the ability to achieve uninterrupted extrusion by effectively adjusting to different bio-ink formulations through meticulous regulation of feeding flow rate, pressure, and extrusion diameter. Notwithstanding the achievement in developing a bio-extrusion printing head capable of precisely and consistently regulating pressure, we faced difficulties pertaining to ink stability, which had an impact on the consistency of printing filaments. The identification of this limitation is recognized as a primary area of interest for future research, with the objective of enhancing the quality and consistency of printed layers. The results of our study provide significant contributions to the understanding of 3D bioprinter nozzle functionality. Additionally, they shed light on the potential progress and obstacles in extrusion technology, emphasizing our dedication to further improving the capabilities of bioprinting technology.