In recent years, significant advancements have been made in flexible electronic systems, particularly in biomedical applications. However, traditional sensors with rigid substrates limit their usage in scenarios where flexibility is required. To address this limitation, flexible adherable sensors have been developed. This study focuses on characterizing the optimal substrate and designing suitable printing patterns for flexible temperature sensors to ensure precise and reliable thermal response for future biomedical applications. Two different geometric patterns, one square and one round” to “Two different geometric patterns, one with square edges and one with round edges, were investigated to assess their influence on sensor sensitivity. Kapton HN substrate was prepared and printed using silver conductive ink. The two circuit patterns were designed and tested to evaluate their impact on sensor performance, particularly regarding sensitivity to temperature variations. Temperature characteristic curves were generated to analyze the relationship between pattern geometry and sensor sensitivity. This research aims to fill the knowledge gaps and contribute to the development of innovative technologies for biomedical monitoring, specifically by exploring the influence of pattern design on sensor performance.