Cyclodextrins (CDs) play a significant role in chemical sensors, particularly in molecular recognition-based analytical techniques like separation science, electroanalysis, single-molecule sensing, spectroscopy, and clinical diagnostics hence, received growing interest among scientists during the past few decades. This chapter focuses on the recent development in the use of CDs for the sensing of several biologically relevant small compounds using fluorescence, electrochemical, and potentiometric response methods. It primarily emphasizes chromophore-linked CDs, metallocyclodextrins, CD-based supramolecular systems, polymer-based CDs, and their role in electrochemical sensing. Additionally, advances in nanotechnology have facilitated the integration of CDs into nanomaterial-based sensor systems, enhancing their functionality and expanding their applications. The chapter also discusses the advantages, disadvantages of CD, and the unique detection mechanisms of CD-functionalized materials in electrochemical sensors, along with recent developments in CD-based electrochemical sensors.