The design of compliant mechanisms requires precise knowledge of the stiffness properties of their flexible segments. When production-induced anisotropy is present, experimental characterization of the flexure hinge’s bending stiffness is crucial, but no test methods specific to flexure hinges are available. This chapter derives and compares novel procedures for testing flexure hinges subjected to large deformations and pure bending loading. The novel methods are the column bending test adapted to flexure hinges (CBT-FH) and the four-point bending test adapted to flexure hinges (FPBT-FH). In addition, a modified tensile test for short-gauge-length specimens is presented. The test methods are validated by comparing calculated deflections and curvatures with those measured by image processing using isotropic materials with well-known properties. The validation shows that the CBT-FH is accurate over the entire deflection range, while the FPBT-FH shows decreased accuracy at large deflections. The CBT-FH achieves a maximum specimen curvature and hinge deflection angle of \(\kappa =0.40\,\text {mm}^{-1}\) and \(\phi ={140}^{\circ}\) , whereas these values are limited in the FPBT-FH to about \(\kappa =0.15\,\text {mm}^{-1}\) and \(\phi ={53}^{\circ}\) . For the isotropic materials examined, the flexural moduli determined by the CBT-FH and FPBT-FH at small strains agree well with the Young’s modulus from the tensile test. While both the CBT-FH and the FPBT-FH methods facilitate precise bending stiffness characterization at small deflections, the CBT-FH is the preferred test method for flexure hinges because it additionally provides accurate test results at large deflections. The test methods validated on isotropic materials form the basis for characterizing anisotropic flexure hinges with inhomogeneous stiffness distributions.