While the literature on gender and tourism in Africa has explored aspects of gender mainstreaming and women’s participation, some gaps remain in comprehensive assessments of tangible outcomes and the sustainability of gender mainstreaming initiatives. Drawing on gender mainstreaming and empowerment theories, this chapter discusses the trends and challenges associated with gender mainstreaming and women’s participation in African rural conservation landscapes. This study utilized an inductive qualitative approach to extract deeper insights from an open-ended examination of secondary data sources on gender mainstreaming and women’s experiences in tourism. The major trends identified included an increase in policy integration and gender mainstreaming, a growth in women’s participation in tourism, an increasing trend in women-led enterprises, increasing financial empowerment among women, limited women’s rights, and the persistence of gender-based inequalities. Challenges include a lack of clear implementation of policies, limited access to credit, lack of training opportunities, and cultural norms and stereotypes. To create more opportunities for women, this chapter recommends expanding access to training and capacity-building programs, diversifying tourism offerings in rural conservation landscapes, leveraging technology and online platforms, improving access to financing and micro-loans, and continuously lobbying for policies that address gender inequalities, foster women’s entrepreneurship, and promote equal access to resources.