Detailed high-resolution palynofacies hydrodynamic and depositional environment, source rock potential and structural hydrocarbon entrapment mechanisms of the outcrop lithostratigraphic sections demonstrates the synergy between the formation, production and hydrocarbon accumulation processes. The analyzed outcroping lithostratigraphic units of the Enugu Formation of the Anambra Basin and Danian units revealed the structural trap style, hydrocarbon production potential framework and migration pathway for oil and gas reserves of the formation for hydrocarbon exploration and exploitation. The well-established lithostratigraphic framework of the Late Campanian to Danian Anambra Basin consists of the Enugu Formation of the Nkporo Group, Manu, Ajali and Nsukka formations. The Anambra Basin is an outcome of the Santonian tectonic event that affected the Benue Trough where the compressional uplift of sediments created a depocenter for the accumulation of the sedimentary sequences of the Anambra Basin formations. The sedimentary characteristic attributes of the Late Campanian to Early Maastrichtian Enugu Formation consist of the carbonaceous shale and sandstone whereas the Danian units is denoted by dark carbonaceous shale and sandstone units. The sedimentary rocks lithostratigraphic units of this rock cycles was illustrated as deposits of regressive and trangressive eustatic sea level fall and rise cycles deposited in fluviotidal, deltaic, shelfal and deep marine settings. The exuberance and hydrodynamics quality of the visual palynofacies analysis is substantiated by the abundance of relatively well-preserved yellow and dark brown amorphous organic matter, marine taxa, opaque particles, with diverse dark brown structured phytoclasts sizes and terrestrial palynomorph. The palynofacies components illustrates dynamic paleoenvironment of transitional marine settings of the inner neritic zone with intermittent outer neritic influences consistent with estuarine, nearshore and freshwater environments based on the normal varied quality and quantity of palynofacies constituent and profusion of land-derived microflora over marine dinoflagellate cysts for the Enugu Formation. The depositional array of the Danian units indicates shallow marine settings with deep marine influenced macro environments, embedded as products of lower and upper deltaic plains with similar variable palynofacies standard inherent in the Enugu Formation. Palynofacies elements suggest a thermally mature Type II–III kerogen indicative of oil and gas prone hydrocarbon for the formations. The kerogen types are conceptualized in the Enugu Formation’s oil seepages and natural gas flare. The natural gas flares reflect the Kerogen Type III (gas prone) palynofacies constituents, while the oil seepages are linked with the Kerogen Type II palynofacies groups. The key structural traps of hanging-wall closures, footwall closures, half graben, horst, collapsed-crest-graben of conjugate fault array, along with infilled and open joint structures within faulted and unfaulted sandstone sequences exhibits the structural hydrocarbon entrapment mechanisms of the Enugu Formation and Danian outcropping sediments. Primary and secondary migration petroleum pathway in sedimentary rock pores and fracture sequences are the key primary means of creating petroleum accumulations in the Anambra Basin. The oil seepages and natural gas flares of the study reveal the structural style elements of the formations as a petroleum system design for hydrocarbon entrapment and preservation model within the Anambra Basin. The structural style complexity, lateral and vertical facies changes, and hydrocarbon prospectivity authenticates the oil seepages and gas flare prediction and subsequent petroleum crude oil and gas drilling pathway as well as exploration and exploitation campaign in the Anambra Basin.