Rye (Secale cereale L.) stands out as one of the most cold-resistant cereal crops, demonstrating lower soil fertility and humidity demands. Temperature fluctuations pose significant challenged to plants and can lead to substantial crop yield losses. Plants response to temperature involve distinct phases, including an initial alarm phase with activated signaling systems and altered protein expression, followed by acclimation, where new proteins are synthesized, and adjustments in membrane density an energy metabolism occur. Continuationof extreme conditions may result in either plant death or adaptation (resistance), while the cessation of stressors initiates a recovery phase. Cytokinins, adenine derivatives, which belong to the key components of the phytohormonal complex, control various plant processes, including cell division, meristem development, photosynthesis, aging, and nutrient uptake in plants. The dynamic modulation of endogenous cytokinins in response to environmental stress depends on multiple factors, among which stress intensity, duration, and plant development stage. Recent research has emphasized the potential of cytokinins in mitigating temperature stress effects, yet there remain significant knowledge gaps regarding the specific role of individual cytokinins, the importance of their conjugation, and interorgan redistribution during different phases of stress responses. Temperature stresses often disrupt cell membrane function and structure, particularly affecting lipids.