The purpose of this study is to investigate how business serviceBusiness services can contribute to the diffusion of a sustainable business ecosystem. It was collected empirical data from thirty different actors that provide services to the automotive business and from industrial actors of the automotive businesses. Through service business, the automotive business players search for achieving sustainability goals. Embedded at interactionInteraction process between the industrial and the service business players emerge several effects, which are spread to their business relationshipsBusiness relationships, occurring a deep sustainability diffusionSustainability diffusion process in business ecosystemSustainability ecosystem. Service businesses players are responsible for: decreasing the difficulties on business processes of automotive business industry; developing green production and greenGreen business activities over the business ecosystem; promoting technological developmentTechnological development; increasing trust and commitment in business relationshipsBusiness relationships due to the interactionInteraction processes, namely related with innovationInnovation; developing a green atmosphere in business relationshipBusiness relationships; and developing a more sustainable business ecosystem.